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In order to be successful in life, I have had to find a very simple way of life that is independent, If you are looking for a software.Improved molecular methods for detection of Campylobacter jejuni and Campylobacter coli in food.
To compare the specificity, sensitivity and cost efficiency of four published PCR methods for the detection of Campylobacter jejuni and Campylobacter coli in food. A total of 462 food samples (225 chicken meat, 218 cattle and 59 buffalo meat) were collected from different farms in East and West Bengal, India. Culture on Campylobacter Blood Agar was carried out for the detection and isolation of C. jejuni and C. coli. The isolates were confirmed as C. jejuni and C. coli by PCR, using C. jejuni- and C. coli-specific primers. The species-specificity of four PCR methods for detection of C. jejuni and C. coli was 100% in chicken and buffalo meat samples (177/177) and 99.4% in cattle meat samples (139/140). The number of positive samples by PCR and culture from different sources (n = 462) was 164 (35.25%). The mean isolation rate of C. jejuni from chicken meat and cattle meat was 29.67% and 35.18%, respectively, while the prevalence of C. coli was 7.8% in chicken meat and 1.85% in cattle meat. Among the four methods tested, the PCR-based methods were cost efficient and allowed rapid detection of C. jejuni and C. coli. More sensitive and efficient methods are required for the accurate, rapid and sensitive detection of C. jejuni and C. coli in food. The species-specific primers used in these methods provide positive results with specific sequences of different strains of C. jejuni and C. coli. The cost was low with a single set of primers. The methods are useful for rapid detection of C. jejuni and C. coli in field conditions with sufficient sensitivity and specificity.Q:
Accessing properties of an xml node programmatically using VS2008
I’m trying to create an automation test tool using VS2008 (C#). I need to be able to access the properties of an XML node of type System.Xml.XmlElement. I’ve tried accessing the values of the properties as below:
elem = (System.
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