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Stellar Windows Live Mail to PST Converter is a tool that enables you to convert Windows Live Mail to. Desktop including Microsoft Outlook, Outlook Express, Microsoft.DESCRIPTION: (Applicant’s Abstract) Oxygen-free radical mediated processes result in biochemical damage to nucleic acids and proteins. DNA is the major target of damage caused by reactive oxygen species. Nucleic acid bases such as guanine, 8-oxo-guanine and 8-oxo-adenine are the common lesions produced in DNA. While 8-oxo-guanine and its photoisomerization products are generally removed by the cellular repair machinery and thus are not mutagenic, 8-oxo-adenine is removed slowly and is a mutagenic lesion. In addition, 8-oxo-adenine can be converted into apurinic or apyrimidinic (AP) sites, an unusual DNA lesion that requires an enzymatic repair mechanism. This proposal is concerned with the mechanisms of formation, repair and the mutagenicity of these uncommon adenine lesions. The enzyme DNA glycosylase from bacterium N- tlepton phosphate lyase (Nth) recognizes and removes the damaged base adenine, producing an AP site. We will use novel Nth mutants and mechanistic probes to test a model for repair of AP sites and other lesions. Mutants defective for glycosylase repair will also be isolated to establish whether or not the lesion may be bypassed. Genetic and chemical evidence suggests that the enzyme OGG1 participates in AP site repair. This hypothesis will be tested by biochemical assays. Finally, studies will be initiated to determine whether two members of the base excision repair (BER) family participate in nucleic acid repair and, if so, if their mechanisms are similar to those of Nth. If not, the consequences of BER error-prone reactions will be assessed. Together, these studies should contribute to our understanding of the mechanisms by which nucleic acids are repaired in cells. The design of this proposal complements the projects in the program in that data from the Nth project will provide the tools to investigate the repair of AP sites.Help
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